QX200™ Droplet Digital™ PCR System

The QX200 Droplet Digital PCR (ddPCR™) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.

Benefits of the QX200 Droplet Digital PCR System

  • Most precise and sensitive digital PCR solution for a wide variety of applications
  • Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
  • Flexible assay setup — scalable for high sensitivity or high throughput
  • Simple and easy-to-use workflow with 96-sample throughput
  • Droplet partitioning by the QX200 Droplet Digital technology reduces bias from amplification efficiency and PCR inhibitors
  • Convenient assay design — standard curves are not required

 

Applications of the QX200 Droplet Digital PCR System

  • Cancer biomarker studies and copy number variation
    Measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution.
  • Pathogen detection
    Employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring.
  • Next generation sequencing
    Perform accurate quantification and qualification of NGS libraries without the use of a standard curve.
  • Gene expression analysis
    Achieve reliable and reproducible measurements of small fold changes for low abundance of mRNA and miRNA.
  • Environmental monitoring
    Test a wide variety of environmental samples like soil and water using the QX200 System.
  • Food testing
    Perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods.

 

 Specifications

 

Starting sample size, µl

20

QX200 droplet generator capacity

1–8 samples/cartridge

Droplets per 20 µl sample

20,000

QX200 droplet reader capacity

1–96 samples

Sample illumination

Light-emitting diodes

Sample detection

Multi-pixel photon counter

Detection channels

FAM (EvaGreen), HEX (VIC)

Linear dynamic range

5 orders of magnitude

Precision

±10%

Droplets per 96-well plate, million

~1.5

QX200 droplet generator dimensions (W x D x H)

28 x 36 x 13 cm (11 x 14 x 5")

QX200 droplet reader dimensions (W x D x H)

66 x 52 x 29 cm (26 x 20 x 11")