The QX200 Droplet Digital PCR (ddPCR™) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
Benefits of the QX200 Droplet Digital PCR System
- Most precise and sensitive digital PCR solution for a wide variety of applications
- Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
- Flexible assay setup — scalable for high sensitivity or high throughput
- Simple and easy-to-use workflow with 96-sample throughput
- Droplet partitioning by the QX200 Droplet Digital technology reduces bias from amplification efficiency and PCR inhibitors
- Convenient assay design — standard curves are not required
Applications of the QX200 Droplet Digital PCR System
- Cancer biomarker studies and copy number variation
Measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. - Pathogen detection
Employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring. - Next generation sequencing
Perform accurate quantification and qualification of NGS libraries without the use of a standard curve. - Gene expression analysis
Achieve reliable and reproducible measurements of small fold changes for low abundance of mRNA and miRNA. - Environmental monitoring
Test a wide variety of environmental samples like soil and water using the QX200 System. - Food testing
Perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods.
Specifications |
|
Starting sample size, µl |
20 |
QX200 droplet generator capacity |
1–8 samples/cartridge |
Droplets per 20 µl sample |
20,000 |
QX200 droplet reader capacity |
1–96 samples |
Sample illumination |
Light-emitting diodes |
Sample detection |
Multi-pixel photon counter |
Detection channels |
FAM (EvaGreen), HEX (VIC) |
Linear dynamic range |
5 orders of magnitude |
Precision |
±10% |
Droplets per 96-well plate, million |
~1.5 |
QX200 droplet generator dimensions (W x D x H) |
28 x 36 x 13 cm (11 x 14 x 5") |
QX200 droplet reader dimensions (W x D x H) |
66 x 52 x 29 cm (26 x 20 x 11") |