CFX96 Touch™ Real-Time PCR Detection System

The CFX96 Touch Real-Time PCR Detection System is a flexible and precise real-time PCR instrument. Its unsurpassed thermal cycler performance and innovative optical design produce accurate, reliable data. The powerful, yet intuitive software accelerates every step of your real-time PCR research, shortening the time between getting started and obtaining great results.

Modular thermal cycler platform, includes C1000 Touch™ Thermal Cycler Chassis, CFX96™ Optical Reaction Module, CFX Maestro™ Software, qbase+ Software license, cables, reagents, consumables

Use the CFX96 Touch Real-Time PCR Detection System for precise, reproducible real-time PCR. The system is composed of the CFX96 Optical Reaction Module, the C1000 Touch Thermal Cycler Chassis, SYBR® Green Supermix, 96-well PCR plates and plate seals.

The module is a member of the family of interchangeable reaction modules that can be used with the C1000 Touch Thermal Cycler Chassis for PCR and real-time PCR. All modules can be quickly inserted and removed from the cycler chassis without tools.

Features and Benefits

• Detection of up to 5 targets per well, plus a channel dedicated to singleplex FRET
• Protocol autowriter that generates an optimal protocol for your reaction components
• Thermal gradient feature that identifies optimal annealing temperature in a single run
• CFX Maestro Software and qbase+ Software for reliable validation, data analysis, and export of RDML files for conformance with MIQE guidelines

The CFX96 Touch™ Real-Time PCR Detection System has six independently controlled thermal electric units providing even, precisely controlled temperatures at all times during the run, including ramping. The reduced mass of the honeycomb block provides fast ramping and reduced settling time (time to achieve thermal uniformity).

The optical system, with six filtered LEDs and six filtered photodiodes, collects data from all wells, detecting up to five targets per well. For single-color FAM and SYBR Green I, the fast scan option reads single-channel fluorescence in all 96 wells in just 3 seconds. A channel with an LED filter–photodiode combination is dedicated to FRET singleplex experiments.

The large touch-screen interface has intuitive graphical programming and displays data traces in real time. The programmable thermal gradient feature can test eight temperatures at once to quickly determine the optimal annealing temperature, and the protocol autowriter quickly optimizes reactions, generating an optimal protocol based on your polymerase, primers, and product length.

With the CFX96 Touch System you can:

• Get great results right away — quick installation and factory-calibrated optics let you set up the system in seconds
• Fit experiments into your schedule — fast thermal cycling produces results in <30 min
• Save research time — thermal gradient feature lets you optimize reactions in a single experiment
• Minimize sample and reagent usage — perform up to 5-target multiplexing and use low sample volumes
• Rely on performance — innovative technology with long-lasting LEDs and solid-state components provides maximum reliability and optimal quantitative results
• Analyze results when and where you want — receive email notification with an attached data file when a run is finished
• Configure the system to fit your laboratory needs — run without a computer, run up to 4 instruments from 1 computer, or integrate with the CFX Automation System II for higher throughput